CAR T-cell therapy uses the target specificity of antibody therapy to direct the cytotoxic immune response3

The autologous anti-CD19 CAR T-cell therapy, TECARTUS, is individually prepared from each patient’s T cells in a 6-step process2:



Obtain peripheral blood mononuclear cells

T cells


Isolate T cells to reduce the likelihood that circulating tumor cells drive the exhaustion of CAR T cells

anti-CD3 and anti-CD28 antibodies + IL-2


Activate T cells using anti-CD3 and anti-CD28 antibodies + IL-2

anti-CD19 CAR transgene


Insert anti-CD19 CAR transgene

CAR T cell expansion

T Cell Expansion

In cell culture, expand the number of CAR T cells to achieve dose

CAR T cells in suspension for infusion


Formulate CAR T cells into suspension for infusion

CAR=chimeric antigen receptor; IL=interleukin.

References: 1. U.S. FDA approves Kite's TECARTUS®, the first and only CAR T treatment for relapsed or refractory mantle cell lymphoma. News release. Kite, a Gilead Company; July 24, 2020. Accessed March 4, 2021. 2. TECARTUS® (brexucabtagene autoleucel). Prescribing information. Kite Pharma, Inc; 2021. 3. Feins S, Kong W, Williams EF, Milone MC, and Fraietta JA. An introduction to chimeric antigen receptor (CAR) T-cell immunotherapy for human cancer. Am J Hematol. 2019;94(suppl 1):S3-S9.